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Image Search Results
Journal: Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation
Article Title: Host Basophils are Dispensable for Induction of Donor Th2 Differentiation and Severity of Experimental Graft-versus-Host Disease
doi: 10.1016/j.bbmt.2011.08.013
Figure Lengend Snippet: (A) Indicated numbers of in vitro IL-3-induced bone marrow B6 basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (DCs, CD11c+) and 4×105 BALB/c CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (B) Indicated numbers of in vitro IL-3-induced BALB/c bone marrow basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (CD11c+) and 4×105 B6 CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (C) Flow cytometric analysis of B6 and BALB/c bone marrow DCs and basophils. MHC class II (I-Ab for B6, I-Ad/I-Ed for BALB/c), CD40, CD80 and CD86 expression levels on CD11c+ cells (DCs, top row) and DX5+ cells (basophils, bottom row) are shown. Numbers shown in each histogram indicate % positivity of the surface marker. (D) Flow cytometric analysis of control hamster mAb (left) or MAR-1 (right) treated BALB/c mouse peripheral blood. Mice were treated with 10 μg of control mAb or MAR-1 three times every 24 hours and peripheral blood samples were corrected from the mice and analyzed. Numbers shown in dot plots indicate % of DX5+FcεRI+ cells.
Article Snippet: For the generation of bone marrow basophils, bone marrow cells were cultured for 9–10 days in RPMI1640 media supplemented with 10% FCS and 10 ng/ml
Techniques: In Vitro, Cell Culture, Expressing, Marker