Journal: Cells

Article Title: In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells

doi: 10.3390/cells11142138

Figure Lengend Snippet: Comparison of the morphology of native hAECs in primary culture and in the control group as well as those differentiated in EGF- and HGF-supplemented media. Magnification—100×; scale—100 µm. In red frames: magnification—200×; scale—50 µm.

Article Snippet: After this time, the cells were passaged and placed in DMEM/F12 differentiation medium no. 1, supplemented with 10% KSOR, 1% NEAA, 1% L-Glut, 1% pen/strep, 1% DMSO (Sigma-Aldrich), and 100 ng/mL EGF or 100 ng/mL HGF (Peprotech), depending on the test group.

Techniques:

Journal: Cells

Article Title: In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells

doi: 10.3390/cells11142138

Figure Lengend Snippet: ( A ) The cell count and viability of hAECs in the control culture (C) and in the test groups (EGF and HGF) expressed as the percentage of viable cells—negative for annexin and propidium iodide; cells in early apoptosis—annexin-positive and propidium-iodide-negative cells; and dead cells—positive for both dyes. The mean number of seeded cells in each of the test groups was 3.7 million. * = statistically significant relative to control cells at 72 h ( p < 0.05); n = 3. ( B ) The representative flow-cytometry histogram taken from control group at 72 h of culture shows the distribution of alive (LL), early apoptotic (LR), and late apoptotic/necrotic cells (UR). Left picture—unstained cells, right picture—stained cells.

Article Snippet: After this time, the cells were passaged and placed in DMEM/F12 differentiation medium no. 1, supplemented with 10% KSOR, 1% NEAA, 1% L-Glut, 1% pen/strep, 1% DMSO (Sigma-Aldrich), and 100 ng/mL EGF or 100 ng/mL HGF (Peprotech), depending on the test group.

Techniques: Cell Counting, Flow Cytometry, Staining

Journal: Cells

Article Title: In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells

doi: 10.3390/cells11142138

Figure Lengend Snippet: Percentage of CD44+, CD73+, CD90+, CD105+, and SSEA-4+ in the control culture (C) and subjected to differentiation (EGF and HGF). Statistically significant differences ( p < 0.05) compared with group (C) at * = 72 h, ** = 15 days, and ## = 18 days; n = 3. The histograms present representative samples of fluorescence spectra in flow cytometry. UC—unstained cells; PC—stained cells.

Article Snippet: After this time, the cells were passaged and placed in DMEM/F12 differentiation medium no. 1, supplemented with 10% KSOR, 1% NEAA, 1% L-Glut, 1% pen/strep, 1% DMSO (Sigma-Aldrich), and 100 ng/mL EGF or 100 ng/mL HGF (Peprotech), depending on the test group.

Techniques: Fluorescence, Flow Cytometry, Staining

Journal: Cells

Article Title: In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells

doi: 10.3390/cells11142138

Figure Lengend Snippet: Expression of marker genes of pluripotency: OCT-4 and SOX2 ; endoderm: SOX17 ; ectoderm: EN2 ; mesoderm: DES ; haematopoietic cells: CD45 . Genes of endoderm-derived cell lineages— AFP , ALB , GSTA1 , GSTP1 , CYP3A4 , and CYP7A1— in the control group and in test groups assessed by the RT-PCR method. C—control; EGF and HGF—test groups; n = 3.

Article Snippet: After this time, the cells were passaged and placed in DMEM/F12 differentiation medium no. 1, supplemented with 10% KSOR, 1% NEAA, 1% L-Glut, 1% pen/strep, 1% DMSO (Sigma-Aldrich), and 100 ng/mL EGF or 100 ng/mL HGF (Peprotech), depending on the test group.

Techniques: Expressing, Marker, Derivative Assay, Reverse Transcription Polymerase Chain Reaction

Journal: Cells

Article Title: In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells

doi: 10.3390/cells11142138

Figure Lengend Snippet: Detection of such glycogen presence in the test groups (PAS reaction). The colour reaction was positive in the EGF and HGF groups and negative in fibroblasts (negative control) and control hAECs (C). Magnification 100×.

Article Snippet: After this time, the cells were passaged and placed in DMEM/F12 differentiation medium no. 1, supplemented with 10% KSOR, 1% NEAA, 1% L-Glut, 1% pen/strep, 1% DMSO (Sigma-Aldrich), and 100 ng/mL EGF or 100 ng/mL HGF (Peprotech), depending on the test group.

Techniques: Negative Control